Marcia Brady

CRISPR/Cas9 PlatformCB is a professional genetic editing biotechnology company that provides transgenic mouse models for biomedical research and drug discovery. We will provide you with a one-stop-shop from experimental design strategies to successful mouse model generation.

The overexpression of proteins is critical for many applications, including studying the function of the gene, and in functional assays and screens. Mammalian cells offer advantages in post-translational modification and protein.

CRISPR/Cas9 PlatformCB is a professional genetic editing biotechnology company that provides transgenic mouse models for biomedical research and drug discovery.

QVirus Platform offers a comprehensive portfolio of oncolytic VACV services for the efficient design, development, manufacturing and analytical testing of virus vector products. So far, we have developed an efficient way to generate recombinant VACVs, including inserting and/or deleting GOI(s) in VACVs. We also provide customized oncolytic vaccinia virus and proof-of-concept validation study for the oncolytic virus.

As a global leader in VLP technology services, Creative Biogene leverages over a decade of technical expertise, international GMP production qualifications, and a comprehensive service system to provide end-to-end solutions for research institutions and pharmaceutical companies—from basic research to industrial production, accelerating the clinical translation and commercialization of innovative therapies.

Creative Biogene, leveraging cutting-edge virology technology platforms, provides pseudovirus construction and customization services covering various viral families. These services support viral function research, vaccine evaluation, neutralizing antibody detection, and antiviral drug screening. Our pseudovirus technology offers a safe and efficient solution for your research and development work.

Creative Biogene specializes in providing high-quality adenovirus vector services, encompassing the construction, amplification, and purification of replication-deficient adenoviruses and helper-dependent adenoviruses (HDAd), supporting various serotypes including Ad5 and Ad5/F35. Leveraging our advanced QVirus™ platform, we have established mature and stable methodologies for vector construction, in vitro recombination, cellular packaging, recombinant adenovirus amplification, and gradient ultracentrifugation purification, complemented by systematic quality control and identification processe

Miniature editors play a crucial role in genomic editing and gene therapy, particularly in addressing human genetic diseases caused by base mutations. Currently, approximately 60% of known human genetic diseases are attributed to base mutations. Traditional CRISPR technology poses risks such as double-stranded DNA breaks, whereas base editors can efficiently convert single bases at target sites without such breaks.

Cell models play a crucial role in drug screening. Through rapid and precise efficacy assessment and simulation of complex disease environments, they effectively identify potential drug candidates. These models not only aid in understanding drug mechanisms and effects but also support personalized medicine and precision drug development. This advances translational medical research and provides new scientific foundations and treatment strategies for various diseases.

In recent years, CRISPR technology has emerged as a transformative tool in the field of biology. CRISPR library screening is a high-throughput screening method based on the CRISPR/Cas9 gene editing system, designed to interfere with the genome using a large-scale sgRNA library to discover new drug targets associated with specific phenotypes or biological processes. This technology employs precisely designed sgRNA sequences introduced into target cells or organisms, facilitating screening under drug pressure or cell sorting conditions to identify genes closely associated with the target phenoty

GFP Reporter Cell Line-HT1080 is engineered to stably overexpress GFP reporter gene. It is an ideal positive control for use in fluorescence assays. In addition, HT1080 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using optical imaging.

Luc Reporter Cell Line-B16F10 is engineered to stably overexpress luciferase reporter gene. It is an ideal positive control for use in bioluminescence assays. In addition, B16F10 cells can form metastases in mouse lungs. The in vivo growth of metastases can be monitored using bioluminescent imaging.

SW480 was derived from colon of a 50 year-old male with colorectal adenocarcinoma. SW480 is negative for CSAp (CSAp-) and colon antigen 3 and are positive for keratin by immunoperoxidase staining. There is a G to A mutation in codon 273 of the p53 gene resulting in an Arg to His substitution and a C to T mutation in codon 309 resulting in a Pro to Ser substitution. SW480 cells express elevated levels of p53 protein. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. N-myc oncogene expression was not detected. Matrilysin, a metalloproteinase associate

Creative Biogene has long-standing experience in conducting preclinical zebrafish model studies of cardiovascular disease. We offer consultancy and preclinical zebrafish models of cardiovascular disease including hypertension, arrhythmia, heart failure and atherosclerosis, etc. Similar to other animal models, wild-type zebrafish do not spontaneously develop cardiovascular diseases mimicking the human's conditions. Therefore, most of the current models rely on chemical interventions or genetic modifications.

Creative Biogene has established more than 50 genetically engineered zebrafish models of human cancer that closely resemble their human counterparts at the histological and/or genomic levels. Our zebrafish cancer models have accelerated the discovery of new mechanisms driving human cancers and identified new drugs for clinical trials. By using a combination of chemical treatment, genetic technology, and tumor cell xenotransplantation, the vast majority of human tumors can be modeled in zebrafish.

Over the past two decades, extensive collections of zebrafish lines carrying mutations in genes involved in early embryonic development have been generated—some of these mutations in genes important for eye formation and associated with eye malformations in humans. Besides, many transgenic lines have been generated at Creative Biogene to enable the close monitoring of organogenesis and the manipulation of gene activity in a tissue-directed way. We mainly use the three approaches to abrogate gene function in zebrafish: stable lines carrying mutations (mutants); morpholino-based knock-down of ge

The CRISPR/Cas9 system is a very versatile tool for a wide variety of genetic changes, including deletions, point mutations, and insertions. The Cas9 nuclease contains two active sites. When both sites are active, Cas9 binds to a guide RNA molecule and causes targeted double-strand breaks (DSBs) in genomic DNA. The CRISPR system can also be used due to the presence of donor DNA molecules homologous to the target region. Specific nucleotide modifications are introduced into the sequence of interest during homologous recombination. Compared to traditional methods, the CRISPR-Cas9 system has significant advantages, including lower cost, shorter timeline and the ability to simultaneously change multiple genes.

CRISPRi expands the potential applications of CRISPR gene editing technology and offers new tools for gene regulation research. Creative Biogene CRISPR/Cas9 PlatformCB provides researchers worldwide with a variety of highly active CRISPRi cell lines that effectively inhibit transcription. Our top-notch cell line products allow for precise and reversible gene inhibition, providing valuable insights into gene function and regulatory mechanisms.

The CRISPR/Cas9 system is a very versatile tool for a wide variety of genetic changes, including deletions, point mutations, and insertions. The Cas9 nuclease contains two active sites. When both sites are active, Cas9 binds to a guide RNA molecule and causes targeted double-strand breaks (DSBs) in genomic DNA. The CRISPR system can also be used due to the presence of donor DNA molecules homologous to the target region. Specific nucleotide modifications are introduced into the sequence of interest during homologous recombination. Compared to traditional methods, the CRISPR-Cas9 system has sign

Gamma-retrovirus (γ-retrovirus) is a genus in the Retroviridae family. Two types of γ-retroviruses are widely used in gene therapy which include MoMLV (Moloney Murine Leukemia Virus) and MSCV (Murine Stem Cell Virus). γ-retroviruses can only transduce dividing cells and can promote the efficient transfer of genes into a variety of cell types.

The retroviral products are available from Creative Biogene are derived from MMLV or MSCV. These particles are developed for expressing fluorescent or bioluminescent reporters which can be used for both in vitro and in vivo applications.